Horsifield, who played the Hero Open and Celtic Classic in the British swing has missed the cut on his three US Open appearances
Sam Horsfield of England will miss this week's US Open at Winged Foot after testing positive for Covid-19.
The 24-year-old returned negative last week, but tested positive after arriving in New York for what will become 2020's second major.
Horsfield qualified for the US Open by winning two. of the six events that made up the UK series of the European Tour.
American Scottie Scheffler, 24, has also withdrawn from the tournament after a positive test.
Horsfield and Scheffler, both asymptomatic, have been replaced in the field by Rory Sa bbatini and Branden Grace respectively.
Grace missed the first major of the year, the US PGA Championship, in August after testing positive halfway through the Barracuda Championship.
The field of 144 will be the smallest at a US Open since 1932.